ࡱ> AC@q` RbjbjqPqP .(:: TTTTTTTh0000L4hI$!h7$TTTTTrbTTT E|0&8$0I$.$$T( IhhhD hhhhhhTTTTTT TECHNICAL SERVICE GUIDE: IMMUNOFLUORESCENCE CUSTOMER INFORMATION Name: Phone:Institude:E-mail:PRODUCT INFORMATION Cat. #: Product Name: Lot. #:Purchase Date: Sales Order Number (if bought from Santa Cruz Shanghai Co.ltd):If bought from a reseller, what is the name of the re-seller: PROBLEM AND PREVIOUS EXPERIENCE 1. What is the specific problem you are experiencing? 2. Did this same vial of product work in the past? What were the results? 3. Did other lots of this product work in the past? Which lots? What were the results? STAINING PATTERN 1. What pattern of staining was expected and what was observed? CONTROLS 1. Has the primary antibody (used in the immunohistochemistry) been used successfully in any other applications (Western Blot, Immunoprecipitation,Immunohistochemistry with different method of fixation) with the same sample? 2. What positive and/or negative controls were done and what were the results? SAMPLES AND FIXATION 1. From which species (animal) was the sample? 2. What type of sample was stained (frozen tissue, paraffin-embedded tissue, tissue culture cells)? 3. What method of fixation was used (acetone, formalin, methanol)? 4. Was Antigen-Retrieval performed? If so, by what method (heat treatment, enzyme digestion)? BLOCKING 1. What were the blocking conditions (blocking solution, species of serum, length of incubation)? PRIMARY ANTIBODY 1. At what dilution(s) was the primary antibody used and what was the diluent? 2. What were the incubation conditions (time, temperature) for the primary antibody? 3. At what temperature has the primary antibody been stored? Has it ever been frozen? SECONDARY ANTIBODY 1. From which company was the secondary antibody obtained and what type of secondary was being used (goat anti-rabbit, rabbit anti-mouse, etc.) and what secondary antibody conjugate was used (biotin, FITC, Rhodamine, Texas Red)? 2. At what dilution(s) was the secondary antibody used and what was the diluent? DETECTION What method of detection was used (ABC Kit, ImmunoCruz Kit, Immunofluorescence)? If a kit was used, was the kit used successfully with other primary antibodies? If performing immunofluorescence, what was the time period between staining and visualization? If performing immunofluorescence, was the filter used appropriate for the fluorochrome? It is appreciated that if images can be attached. 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